diff --git a/core/src/components/input-otp/input-otp.tsx b/core/src/components/input-otp/input-otp.tsx
index 7c46ca59d42..1c84b3c9a42 100644
--- a/core/src/components/input-otp/input-otp.tsx
+++ b/core/src/components/input-otp/input-otp.tsx
@@ -43,6 +43,12 @@ export class InputOTP implements ComponentInterface {
    */
   private isKeyboardNavigation = false;
 
+  /**
+   * The `length` watcher runs before new inputs render, so
+   * `componentDidRender` updates their tab indexes instead.
+   */
+  private updateTabIndexesAfterRender = false;
+
   @Element() el!: HTMLIonInputOtpElement;
 
   @State() private inputValues: string[] = [];
@@ -197,6 +203,12 @@ export class InputOTP implements ComponentInterface {
     this.updateTabIndexes();
   }
 
+  @Watch('length')
+  lengthChanged() {
+    this.initializeValues();
+    this.updateTabIndexesAfterRender = true;
+  }
+
   /**
    * Processes the separators prop into an array of numbers.
    *
@@ -272,6 +284,13 @@ export class InputOTP implements ComponentInterface {
     this.updateTabIndexes();
   }
 
+  componentDidRender() {
+    if (this.updateTabIndexesAfterRender) {
+      this.updateTabIndexesAfterRender = false;
+      this.updateTabIndexes();
+    }
+  }
+
   /**
    * Get the regex pattern for allowed characters.
    * If a pattern is provided, use it to create a regex pattern
@@ -851,7 +870,14 @@ export class InputOTP implements ComponentInterface {
                   tabIndex={index === tabbableIndex ? 0 : -1}
                   value={inputValues[index] || ''}
                   autocomplete="one-time-code"
-                  ref={(el) => (inputRefs[index] = el as HTMLInputElement)}
+                  ref={(el) => {
+                    if (el) {
+                      inputRefs[index] = el as HTMLInputElement;
+                    } else {
+                      // The input was removed, so drop its ref and any after it.
+                      inputRefs.splice(index);
+                    }
+                  }}
                   onInput={this.onInput(index)}
                   onBlur={this.onBlur}
                   onFocus={this.onFocus(index)}
diff --git a/core/src/components/input-otp/test/a11y/input-otp.e2e.ts b/core/src/components/input-otp/test/a11y/input-otp.e2e.ts
index 88b0be04800..7cc5374d8ef 100644
--- a/core/src/components/input-otp/test/a11y/input-otp.e2e.ts
+++ b/core/src/components/input-otp/test/a11y/input-otp.e2e.ts
@@ -46,6 +46,27 @@ configs().forEach(({ title, config }) => {
       await expect(inputBoxes.nth(3)).toHaveAttribute('aria-hidden', 'true');
     });
 
+    test('should update aria-hidden when length increases', async ({ page }, testInfo) => {
+      testInfo.annotations.push({
+        type: 'issue',
+        description: 'https://github.com/ionic-team/ionic-framework/issues/31484',
+      });
+
+      await page.setContent(`<ion-input-otp length="2"></ion-input-otp>`, config);
+
+      const inputOtp = page.locator('ion-input-otp');
+      await inputOtp.evaluate((el: HTMLIonInputOtpElement) => {
+        el.length = 4;
+      });
+
+      const inputBoxes = page.locator('ion-input-otp input');
+
+      await expect(inputBoxes.nth(0)).toHaveAttribute('aria-hidden', 'false');
+      await expect(inputBoxes.nth(1)).toHaveAttribute('aria-hidden', 'true');
+      await expect(inputBoxes.nth(2)).toHaveAttribute('aria-hidden', 'true');
+      await expect(inputBoxes.nth(3)).toHaveAttribute('aria-hidden', 'true');
+    });
+
     test('should update aria-hidden when typing a value', async ({ page }) => {
       await page.setContent(`<ion-input-otp></ion-input-otp>`, config);
 
diff --git a/core/src/components/input-otp/test/basic/input-otp.e2e.ts b/core/src/components/input-otp/test/basic/input-otp.e2e.ts
index 306e72f873d..a135f502f0a 100644
--- a/core/src/components/input-otp/test/basic/input-otp.e2e.ts
+++ b/core/src/components/input-otp/test/basic/input-otp.e2e.ts
@@ -71,6 +71,39 @@ configs({ modes: ['ios'] }).forEach(({ title, config }) => {
       await verifyInputValues(inputOtp, ['1', '2', '3', '4', '5', '6', '7', '8']);
     });
 
+    test('should synchronize the value when length changes', async ({ page }, testInfo) => {
+      testInfo.annotations.push({
+        type: 'issue',
+        description: 'https://github.com/ionic-team/ionic-framework/issues/31484',
+      });
+
+      await page.setContent(`<ion-input-otp length="4" value="1234">Description</ion-input-otp>`, config);
+
+      const inputOtp = page.locator('ion-input-otp');
+      await inputOtp.evaluate((el) => el.setAttribute('length', '2'));
+
+      await verifyInputValues(inputOtp, ['1', '2']);
+    });
+
+    test('should add empty input boxes when length increases', async ({ page }, testInfo) => {
+      testInfo.annotations.push({
+        type: 'issue',
+        description: 'https://github.com/ionic-team/ionic-framework/issues/31484',
+      });
+
+      await page.setContent(`<ion-input-otp length="2" value="12">Description</ion-input-otp>`, config);
+
+      const inputOtp = page.locator('ion-input-otp');
+      await inputOtp.evaluate((el: HTMLIonInputOtpElement) => {
+        el.length = 4;
+      });
+
+      const inputBoxes = page.locator('ion-input-otp input');
+      await expect(inputBoxes).toHaveCount(4);
+
+      await verifyInputValues(inputOtp, ['1', '2', '', '']);
+    });
+
     test('should accept numbers only by default', async ({ page }) => {
       await page.setContent(`<ion-input-otp>Description</ion-input-otp>`, config);
 
@@ -1033,6 +1066,36 @@ configs({ modes: ['ios'], directions: ['ltr'] }).forEach(({ title, config }) =>
       await expect(ionChange).toHaveReceivedEventTimes(1);
     });
 
+    test('should emit ionChange event when blurring with a new value after length decreases', async ({
+      page,
+    }, testInfo) => {
+      testInfo.annotations.push({
+        type: 'issue',
+        description: 'https://github.com/ionic-team/ionic-framework/issues/31484',
+      });
+
+      await page.setContent(`<ion-input-otp length="4">Description</ion-input-otp>`, config);
+
+      const ionChange = await page.spyOnEvent('ionChange');
+
+      const inputOtp = page.locator('ion-input-otp');
+      await inputOtp.evaluate((el: HTMLIonInputOtpElement) => {
+        el.length = 2;
+      });
+      await page.waitForChanges();
+
+      const firstInput = page.locator('ion-input-otp input').first();
+      await firstInput.focus();
+
+      await page.keyboard.type('12');
+
+      // Click outside the input to trigger the blur event
+      await page.mouse.click(0, 0);
+
+      await ionChange.next();
+      await expect(ionChange).toHaveReceivedEventDetail({ value: '12', event: { isTrusted: true } });
+    });
+
     test('should not emit ionChange event when blurring with the same value', async ({ page }) => {
       await page.setContent(`<ion-input-otp value="12">Description</ion-input-otp>`, config);